Possible ameliorative effect of human placental extract on methotrexate-induced nephrotoxicity in albino rats

Abstract Background Methotrexate (MTX) is one of chemotherapeutic drugs that induce several side effects. The present study aimed to investigate the ameliorative effect human placental extract (HPE) against MTX-induced nephrotoxicity in rats. In this study, forty adult male albino rats were equally divided into four groups. Control group: daily injected intraperitoneally with physiological saline (0.5 ml for each rat) 5 days, HPE subcutaneously at a dose level 10.08 mg/Kg b.w/day 2 weeks, MTX consecutive and (at same dosage group) days time they an exact group), weeks. Twenty-four hours after last treatment, killed blood samples collected determination urea, creatinine, sodium (Na + ) potassium (K levels. Kidney tissues taken histological examination immunohistochemical staining both cysteine-aspartic protease-3 (caspase-3) proliferating antigen Ki-67 (Ki-67) expressions. Results From obtained data, induced through highly significant increase Na K levels compared control group. addition massive alterations, caspase-3 expression decrease observed. On other hand, injection ameliorated comparing Moreover, markedly improved changes resulted from treatment. Conclusions It concluded by MTX.